Italian · Pronunciation Guide
DOO-eh, two distinct syllables, not the one-syllable "doo" of English "due." In IPA: /ˈdu.e/.
Two syllables, du and e, both fully voiced. English "due" looks like a cognate but is only ever one syllable, which is exactly the trap here.
Listen: due
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This word is a textbook example of a spelling false friend: it looks almost identical to an English word that's pronounced completely differently. Reading each Italian letter as its own sound, rather than importing the English word wholesale, gets it right immediately.
Ho due fratelli.
I have two brothers.
Listen: Ho due fratelli.
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Unlike "uno," which changes form depending on gender ("un cane," "una casa"), "due" never changes: "due fratelli" (two brothers) and "due sorelle" (two sisters) use the exact same word regardless of what's being counted. That makes it one of the easier numbers to use correctly once its pronunciation is secure.
"Fratelli" itself defaults to the masculine plural even for a mixed-gender group of siblings, so "ho due fratelli" could mean two brothers or one brother and one sister, context usually resolves which.
The single biggest mistake is collapsing "due" into one syllable, borrowing the pronunciation of its English lookalike "due" (as in "the payment is due"). Italian "due" keeps both vowels fully separate and voiced, "DOO-eh," in two clear beats, never merging into a single English-style syllable.
The second slip is adding a y-glide to the u, the way English sometimes pronounces a u after d (as in a careful "duty"). Italian d is dental and the following u is a pure vowel with no glide at all, so the syllable should sound cleaner and more clipped than its English counterpart.